p21 antibody (AA 143-147)

Catalog No. ABIN362755

This anti-p21 antibody is a Rabbit Polyclonal antibody detecting p21 in WB. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for p21 antibody (AA 143-147) (ABIN362755)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-p21 Antibody

Binding Specificity: AA 143-147

Specificity: The antibody detects endogenous level of total p21Cip1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 143-147 (R-Q-T-S-M) derived from Human p21Cip1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-p21 antibody (ABIN362755)

Kanehira, Kikuchi, Ohkouchi, Shibahara, Tode, Santoso, Daito, Ohta, Tamada, Nukiwa: "Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e32185, (2012) (PubMed).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21Cip1

Background: May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

Molecular Weight: 21 kDa

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy