KLF4 antibody (AA 1-170)

Catalog No. ABIN364360

Product Details anti-KLF4 Antibody

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Binding Specificity: AA 1-170

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human KLF4 (1-170aa) purified from E. coli. NCBI Accession No.: NP_004226.3.

Type of Immunogen: Recombinant protein

Clone: AT4E6

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (20 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4 (KLF4 Products)

Synonyms: EZF antibody, GKLF antibody, Gklf antibody, Zie antibody, KLF4 antibody, biklf antibody, biklf-A antibody, Kruppel like factor 4 antibody, Kruppel-like factor 4 (gut) antibody, Kruppel-like factor 4 antibody, Kruppel-like factor 4 (gut) L homeolog antibody, KLF4 antibody, Klf4 antibody, klf4 antibody, klf4.L antibody

Background: Name/Gene ID: KLF4

Synonyms: KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4

Gene ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance