Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 441-454) antibody

Catalog No. ABIN364387

Product Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Binding Specificity: AA 441-454

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ACADM. This antibody is expected to recognise both reported isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Marmoset, Mouse, Rat, Horse, Opossum (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RLIVAREHIDKYKN from the C-terminus of human ACADM (NP_000007.1, NP_001120800.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Marmoset, Mouse, Rat, Horse, Opossum (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.01 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Alternative Name: MCAD / ACADM

Synonyms: ACAD1 antibody, MCAD antibody, MCADH antibody, AU018656 antibody, acyl-CoA dehydrogenase medium chain antibody, acyl-Coenzyme A dehydrogenase, medium chain antibody, acyl-CoA dehydrogenase, C-4 to C-12 straight chain antibody, ACADM antibody, Acadm antibody

Background: Name/Gene ID: ACADM

Synonyms: ACADM, ACAD1, MCAD, MCADH

Gene ID: 34

NCBI Accession: NP_000007, NP_001120800

UniProt: P11310