APOB antibody (AA 3984-3995)
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- Target See all APOB Antibodies
- APOB (Apolipoprotein B (APOB))
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Binding Specificity
- AA 3984-3995
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Reactivity
- Human, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This APOB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human APOB.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-TDLHLRYQKDKK from an internal region of human APOB (NP_000375.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product APOB Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Band corresponding to the expected size of ApoB100 is seen along with additional bands at lower molecular weights. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- APOB (Apolipoprotein B (APOB))
- Alternative Name
- Apolipoprotein B / APOB (APOB Products)
- Synonyms
- FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody
- Background
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Name/Gene ID: APOB
Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48 - Gene ID
- 338
- NCBI Accession
- NP_000375
- UniProt
- P04114
- Pathways
- Lipid Metabolism
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