SLC22A17 antibody (AA 476-489)

Catalog No. ABIN364414

Product Details anti-SLC22A17 Antibody

Target: SLC22A17 (Solute Carrier Family 22 (Organic Cation Transporter), Member 17 (SLC22A17))

Binding Specificity: AA 476-489

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Pig, Monkey, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC22A17 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SLC22A17. This antibody is expected to recognize both reported isoforms NP_065105.2 and NP_057693.3).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig (100%) Elephant, Rabbit (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PETKRKLLPEVLRD from an internal region of human SLC22A17 (NP_065105.2, NP_057693.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig (100%), Elephant, Rabbit (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL. Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 150kD band in Human Cerebellum lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7kD according to NP_065105.2. The 150kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SLC22A17

Target: SLC22A17 (Solute Carrier Family 22 (Organic Cation Transporter), Member 17 (SLC22A17))

Alternative Name: SLC22A17 (SLC22A17 Products)

Synonyms: 1700094C23Rik antibody, 24p3R antibody, AU041908 antibody, AW555662 antibody, BOIT antibody, Boct antibody, mBOCT antibody, BOCT antibody, NGALR antibody, NGALR2 antibody, NGALR3 antibody, hBOIT antibody, rBOCT antibody, solute carrier family 22 member 17 antibody, solute carrier family 22 (organic cation transporter), member 17 antibody, solute carrier family 22, member 17 antibody, SLC22A17 antibody, Slc22a17 antibody

Background: Name/Gene ID: SLC22A17
Subfamily: Organic cation transporter
Family: Transporter

Synonyms: SLC22A17, 24p3 receptor, 24p3R, BOIT, BOCT, HBOIT, NGALR, NGAL receptor, NGALR2, Lipocalin-2 receptor, MBOCT, NGALR3

Gene ID: 51310

NCBI Accession: NP_065105, NP_057693

UniProt: Q8WUG5

Pathways: Transition Metal Ion Homeostasis