GAD antibody (AA 526-537)

Catalog No. ABIN364418

Product Details anti-GAD Antibody

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Binding Specificity: AA 526-537

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GAD1 / GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (92%) Mouse, Rat, Elephant, Panda, Bat, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PDSPQRREKLHK from an internal region of human GAD1 / GAD67 (NP_000808.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (92%), Mouse, Rat, Elephant, Panda, Bat, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:8000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kD according to Human NP_000808.2 and 66.6kD according to Mouse NP_032103.2). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD67 / GAD (GAD1 Products)

Synonyms: CPSQ1 antibody, GAD antibody, SCP antibody, GAD-67 antibody, cpsq1 antibody, gad1 antibody, gad1-A antibody, gad67 antibody, GAD67 antibody, GAD1 antibody, GLUTAMATE DECARBOXYLASE antibody, GLUTAMATE DECARBOXYLASE 1 antibody, MKP11.30 antibody, MKP11_30 antibody, glutamate decarboxylase antibody, EP10 antibody, GAD25 antibody, GAD44 antibody, Gad-1 antibody, Gad67 antibody, glutamate decarboxylase 1 antibody, glutamate decarboxylase 1 L homeolog antibody, glutamate decarboxylase antibody, glutamate decarboxylase 1b antibody, GAD1 antibody, gad1.1.L antibody, GAD antibody, Gad1 antibody, gad1b antibody

Background: Name/Gene ID: GAD1

Synonyms: GAD1, CPSQ1, GAD-67, Glutamate decarboxylase 1, GAD67, GAD, SCP

Gene ID: 2571

NCBI Accession: NP_000808

UniProt: Q99259