SLC5A1 antibody

Catalog No. ABIN364451

Product Details anti-SLC5A1 Antibody

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC5A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: The antibody detects 73 kD SGLT-1 from human samples and in a lesser extent from mouse and rat samples. A ~50 kD cleavage fragment can also be detected in Jurkat cell lysate.

Purification: Protein A purified

Immunogen: Synthetic peptide surrounding amino acid 594 of human SGLT-1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 30 % glycerol, 0.5 % BSA, 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

References for anti-SLC5A1 antibody (ABIN364451)

Wanes, Naim, Dengler: "Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum." in: Cells, Vol. 10, Issue 11, (2021) (PubMed).

Target Details for SLC5A1

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Alternative Name: SLC5A1 / SGLT1 (SLC5A1 Products)

Synonyms: D22S675 antibody, NAGT antibody, SGLT1 antibody, zgc:66074 antibody, zgc:85782 antibody, SLC5A1 antibody, Sglt1 antibody, solute carrier family 5 member 1 antibody, solute carrier family 5 (sodium/glucose cotransporter), member 1 antibody, SLC5A1 antibody, slc5a1 antibody, Slc5a1 antibody

Background: Name/Gene ID: SLC5A1
Subfamily: Solute:sodium symporter
Family: Transporter

Synonyms: SLC5A1, D22S675, HSGLT1, Na(+)/glucose cotransporter 1, Na+/glucose cotransporter 1, SGLT1, NAGT, Sodium-glucose transporter 1, Sodium/glucose cotransporter 1

Gene ID: 6523

UniProt: P13866