MYL2 antibody (N-Term)

Catalog No. ABIN371883

Product Details anti-MYL2 Antibody

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Binding Specificity: AA 1-166, N-Term

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: The antibody recognizes Human and Rat MYL2 (1-166). Other species not tested.

Characteristics: Synonyms: MLC-2v, MLC-2, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform

Purification: Protein-G affinity chromatography

Immunogen: Recombinant Human MYL2 (1-166 aa) purified from E. coli

Clone: AT3B2

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (5 μg/mL). This MYL2 antibody was validatedfor use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Alternative Name: Myosin Light Chain 2 (MYL2) (MYL2 Products)

Synonyms: CMH10 antibody, MLC2 antibody, 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, MLC-2v antibody, Mlc2v antibody, Mylpc antibody, Mlc-2 antibody, myl2 antibody, zgc:136848 antibody, XMLC2 antibody, cmh10 antibody, mlc2 antibody, G2 antibody, MLC-2A antibody, MLC2V antibody, myosin light chain 2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin, light polypeptide 2, regulatory, cardiac, slow antibody, myosin, light chain 2b, regulatory, cardiac, slow antibody, myosin light chain 2 S homeolog antibody, myosin, light chain 2, regulatory, cardiac, slow antibody, MYL2 antibody, Mylpf antibody, Myl2 antibody, myl2b antibody, myl2.S antibody

Background: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.Synonyms: MLC-2, MLC-2v, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform

Gene ID: 4633

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction