NMDA 1 Receptor antibody (Splice Variant C2)

Catalog No. ABIN372676

Product Details anti-NMDA 1 Receptor Antibody

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Binding Specificity: Splice Variant C2

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDA 1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes the ~120k NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from the NR1 subunit, C2 splice variant insert of Rat NMDA Receptor.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-NMDA 1 Receptor antibody (ABIN372676)

Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, (2001) (PubMed).

Target Details for NMDA 1 Receptor

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Alternative Name: NMDA Receptor 1 (NMDA R1 Products)

Synonyms: CG2902 antibody, DMNMDAR1 antibody, DNMDAR-I antibody, DNmdar antibody, DmelNmdar1 antibody, Dmel\\CG2902 antibody, DrNR1 antibody, NMDA antibody, NMDA R1 antibody, NMDAR1 antibody, NMdar1 antibody, NR1 antibody, NRI antibody, Nmdar antibody, dNR1 antibody, dnmdar-I antibody, nmr antibody, NMDA receptor 1 antibody, Nmdar1 antibody

Background: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1

Gene ID: 24408

NCBI Accession: NP_058706

UniProt: P35439