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NMDA 1 Receptor antibody (Splice Variant C2')

NMDA R1 Reactivity: Human, Rat, Mouse, Cow WB, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN372677
  • Target See all NMDA 1 Receptor (NMDA R1) Antibodies
    NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))
    Binding Specificity
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Splice Variant C2'
    Reactivity
    • 19
    • 16
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Cow
    Host
    • 18
    • 5
    Rabbit
    Clonality
    • 18
    • 5
    Polyclonal
    Conjugate
    • 23
    This NMDA 1 Receptor antibody is un-conjugated
    Application
    • 18
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody recognizes the ~120k NR1 subunit of the NMDA receptor containing the C2' splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2' insert.
    Cross-Reactivity (Details)
    Species reactivity (expected):Bovine and Human.
    Species reactivity (tested):Mouse and Rat.
    Purification
    Affinity Chromatography.
    Immunogen
    Peptide from the NR1 subunit, C2' splice variant insert of rat NMDA Receptor.
    Isotype
    IgG
    Top Product
    Discover our top product NMDA R1 Primary Antibody
  • Application Notes
    Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
    Buffer
    5 mM Ammonium Bicarbonate.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted (in aliquots) at-20 °C.
  • Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

    Song, Kaczmarek: "Modulation of Kv3.1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15582-91, (2006) (PubMed).

  • Target
    NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))
    Alternative Name
    NMDA Receptor 1 (NMDA R1 Products)
    Synonyms
    CG2902 antibody, DMNMDAR1 antibody, DNMDAR-I antibody, DNmdar antibody, DmelNmdar1 antibody, Dmel\\CG2902 antibody, DrNR1 antibody, NMDA antibody, NMDA R1 antibody, NMDAR1 antibody, NMdar1 antibody, NR1 antibody, NRI antibody, Nmdar antibody, dNR1 antibody, dnmdar-I antibody, nmr antibody, NMDA receptor 1 antibody, Nmdar1 antibody
    Background
    The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1
    Gene ID
    24408
    NCBI Accession
    NP_058706
    UniProt
    P35439
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