PLK1 antibody (pThr210)

Catalog No. ABIN372703

This Rabbit Polyclonal antibody specifically detects PLK1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Xenopus laevis, Dog, Cow, Zebrafish (Danio rerio) and Non-Human Primate.

Quick Overview for PLK1 antibody (pThr210) (ABIN372703)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat, Xenopus laevis, Dog, Cow, Zebrafish (Danio rerio), Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK1 Antibody

Binding Specificity: pThr210

Specificity: This antibody recognizes the ~66k PLK protein phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat and Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase