×
For best experience we recommend to activate Javascript in your browser.
RAF1 antibody (pSer642)
RAF1
Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Non-Human Primate
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-RAF1 Antibody
(hide)
Target
See all RAF1 Antibodies
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Binding Specificity
All epitopes for RAF1 antibodies
pSer642
Reactivity
All reactivities for RAF1 antibodies
Human, Mouse, Rat, Cow, Chicken, Dog, Non-Human Primate
Host
All hosts for RAF1 antibodies
Rabbit
Clonality
All clonalities for RAF1 antibodies
Polyclonal
Conjugate
All conjugates for RAF1 antibodies
This RAF1 antibody is un-conjugated
Application
All applications for RAF1 antibodies
Western Blotting (WB)
Specificity
This antibody recognizes the ~74k Raf-1 protein phosphorylated at Ser642.
Cross-Reactivity (Details)
Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates. Species reactivity (tested):Rat.
Purification
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf1.
Isotype
IgG
Top Product
Discover our top product RAF1 Primary Antibody
Alternatives
(show)
Application Details
(hide)
Application Notes
Western blot: 1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
(hide)
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Target Details for RAF1
(hide)
Target
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Alternative Name
RAF1 (RAF1 Products )
Synonyms
CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
Background
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID
24703
NCBI Accession
NP_036771
UniProt
P11345
Pathways
MAPK Signaling , RTK Signaling , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , cAMP Metabolic Process , Stem Cell Maintenance , Hepatitis C , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling , BCR Signaling
Recently viewed
(hide)