MAPT antibody (pSer416)

Catalog No. ABIN372727

This Rabbit Polyclonal antibody specifically detects MAPT in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog and Non-Human Primate.

Quick Overview for MAPT antibody (pSer416) (ABIN372727)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human, Rat, Mouse, Cow, Dog, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPT Antibody

Binding Specificity: pSer416

Specificity: Specific for ~59, 65, 68k Tau protein phosphorylated at Ser416.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Canine, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography via sequential chromatography on phospho- and dephospho-peptide affinity columns

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of Rat MAPT/TAU.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT / tau

Background: Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer's disease (AD), Pick's disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

Gene ID: 10116

UniProt: P19332

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size