LY6C + LY6G antibody
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- Target
- LY6C + LY6G
- Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- Mouse Ly-6G/Ly-6C, Mr 21-25 kDa
- Characteristics
- Rat Anti-Mouse Ly-6G/Ly-6C-LE/AF
- Purification
- Purified
- Endotoxin Level
- Low endotoxin
- Immunogen
- Unknown
- Clone
- RB6-8C5
- Isotype
- IgG2b
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg of purified immunoglobulin in 1.0 mL of PBS
- Preservative
- Azide free
- Handling Advice
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- Storage
- -20 °C
- Storage Comment
- Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
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- Target
- LY6C + LY6G
- Alternative Name
- Ly-6G/Ly-6C
- Background
- Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. 1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. 2 In the periphery, Ly-6G is expressed predominantly on neutrophils.
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