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Phosphotyrosine antibody (FITC)

ELISA Host: Mouse Monoclonal PY20 FITC
Catalog No. ABIN376118
  • Target See all Phosphotyrosine products
    Phosphotyrosine
    Reactivity
    Please inquire
    Host
    • 84
    • 23
    • 1
    Mouse
    Clonality
    • 95
    • 13
    Monoclonal
    Conjugate
    • 37
    • 16
    • 10
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Phosphotyrosine antibody is conjugated to FITC
    Application
    • 75
    • 53
    • 32
    • 31
    • 30
    • 21
    • 20
    • 17
    • 13
    • 9
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA
    Specificity
    Tyrosine-phosphorylated proteins
    Characteristics
    Mouse Anti-Phosphotyrosine-FITC
    Purification
    Purified
    Immunogen
    Phosphotyrosine conjugated to carrier proteins
    Clone
    PY20
    Isotype
    IgG2b
  • Application Notes
    • Applications: ELISA - Quality tested , FLISA - Quality tested WB - Reported in literature , IP - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , FC - Reported in literature , Microarray - Reported in literature , Purification - Reported in literature
    • Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:1,000 - 1:4,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate ≤ 1 g/mL
    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg in 1.0 mL of PBS/Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    Phosphotyrosine
    Abstract
    Phosphotyrosine Products
    Target Type
    Amino Acid
    Background
    Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine.
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