AKT1 antibody (pSer473)

Catalog No. ABIN377342

The Sheep Polyclonal anti-AKT1 antibody has been validated for WB and IHC. It is suitable to detect AKT1 in samples from Human, Mouse and Rat.

Quick Overview for AKT1 antibody (pSer473) (ABIN377342)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AKT1 Antibody

Binding Specificity: pSer473

Specificity: Specific for AKT1, AKT2, AKT3.

Purification: Purified

Immunogen: Synthetic peptide from the phospho S473 of mouse AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein. The antigen is homologous in rat, human and chicken AKT1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, WB

Usage: IHC: Antigen retrieval is essential for use on paraffin sections.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized. Centrifuge to remove any insoluble material

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1 / AKT

Background: Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect