His Tag antibody (HRP)

Details for Product No. ABIN387701, Supplier: Log in to see
Antigen
Reactivity
Tag
624
28
15
5
4
1
Host
Mouse
413
189
27
23
8
8
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
57
46
37
17
13
13
10
10
10
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
569
405
165
109
83
82
79
27
13
11
8
7
6
6
5
5
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand THE™
Immunogen A synthetic peptide HHHHHH coupled - KLH
Sequence HHHHHH
Clone 6G2A9
Isotype IgG
Specificity THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Characteristics This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Purification Protein A affinity column
Target Type Tag
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Supplier Images
 image for anti-His Tag antibody (HRP) (ABIN387701) Lane 1: MarkerLane 2: 10 ng Multiple Tag Cell Lysate fusion protein (ABIN1536505) Lan...
Product cited in: Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, 2015 (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, 2015 (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, 2014 (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, 2014 (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, 2013 (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, 2012 (PubMed).

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