p21 antibody (pThr57)

Catalog No. ABIN389613

This anti-p21 antibody is a Rabbit Polyclonal antibody detecting p21 in IHC (p) and DB. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for p21 antibody (pThr57) (ABIN389613)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Clone: RB7643

Product Details anti-p21 Antibody

Binding Specificity: pThr57

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This P21CIP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T57 of human P21CIP1.

Isotype: Ig Fraction

Application Details

Application Notes: IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-p21 antibody (ABIN389613)

Colleoni, Paternot, Pita, Bisteau, Coulonval, Davis, Raspé, Roger: "JNKs function as CDK4-activating kinases by phosphorylating CDK4 and p21." in: Oncogene, Vol. 36, Issue 30, pp. 4349-4361, (2017) (PubMed).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21Cip1

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Molecular Weight: 18119

Gene ID: 1026

NCBI Accession: NP_000380, NP_001207706, NP_001207707, NP_510867

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy