Caspase 8 antibody (C-Term)

Catalog No. ABIN390474

This anti-Caspase 8 antibody is a Rabbit Polyclonal antibody detecting Caspase 8 in WB and FACS. Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Caspase 8 antibody (C-Term) (ABIN390474)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RB18993

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 432-461, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CASP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human CASP8.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Caspase 8 antibody (ABIN390474)

Lin, Huang, Lin, Lin: "p-Cresol mediates autophagic cell death in renal proximal tubular cells." in: Toxicology letters, Vol. 234, Issue 1, pp. 20-9, (2015) (PubMed).

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background: CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

Molecular Weight: 55391

Gene ID: 841

NCBI Accession: NP_001073593, NP_001073594, NP_001219, NP_203519, NP_203520, NP_203522

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades