CDK4 antibody (C-Term)

Catalog No. ABIN391751

The Rabbit Polyclonal anti-CDK4 antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect CDK4 in samples from Human. There are 3+ publications available.

Quick Overview for CDK4 antibody (C-Term) (ABIN391751)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB03154

Product Details anti-CDK4 Antibody

Binding Specificity: AA 273-305, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CDK4 antibody (ABIN391751)

Ling, Song, Fan, Feng, Liu, Yang, Wang, Li, Tian, Zhao, Liu, Huang, Hou, Xu, Shi, Li: "Combination of metformin and sorafenib suppresses proliferation and induces autophagy of hepatocellular carcinoma via targeting the mTOR pathway." in: International journal of oncology, Vol. 50, Issue 1, pp. 297-309, (2017) (PubMed).

Patel, Asbach, Shteyn, Gomez, Coltoff, Bhuyan, Tyner, Wagner, Blain: "Brk/Protein tyrosine kinase 6 phosphorylates p27KIP1, regulating the activity of cyclin D-cyclin-dependent kinase 4." in: Molecular and cellular biology, Vol. 35, Issue 9, pp. 1506-22, (2015) (PubMed).

Ling, Feng, Ke, Fan, Li, Li, Dong, Wang, Xu, Li, Wang: "Metformin inhibits proliferation and enhances chemosensitivity of intrahepatic cholangiocarcinoma cell lines." in: Oncology reports, Vol. 31, Issue 6, pp. 2611-8, (2014) (PubMed).

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

Molecular Weight: 33730

Gene ID: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size