ARAF antibody (AA 256-286)

Catalog No. ABIN392244

This anti-ARAF antibody is a Rabbit Polyclonal antibody detecting ARAF in WB and IHC (p). Suitable for Human.

Quick Overview for ARAF antibody (AA 256-286) (ABIN392244)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB03325

Product Details anti-ARAF Antibody

Binding Specificity: AA 256-286

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A-RAF (ARAF1)

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Molecular Weight: 67585

Gene ID: 369

NCBI Accession: NP_001243125, NP_001243126, NP_001645

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C