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BDNF antibody (C-Term)

Name: BDNF antibody (C-Term)
SKU: ABIN392515
Price: 451 USD
Availability: InStock

BDNF Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14839 unconjugated

All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 μg per lane. (BDNF antibody (C-Term))

Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BDNF antibody (C-Term))

IHC (p)

Confocal immunofluorescent analysis of BDNF Antibody (C-term) (ABIN392515 and ABIN2842076) with NCI- cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (BDNF antibody (C-Term))

Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). (BDNF antibody (C-Term))

FACS

4 images

(1 reference)

Catalog No. ABIN392515

Datasheet as PDF

Target See all BDNF Antibodies

BDNF (Brain-Derived Neurotrophic Factor (BDNF))
Binding Specificity
16

15

11

10

8

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 206-236, C-Term
Reactivity
117

62

59

14

10

10

9

6

5

5

5

4

3

3

1
Human
Host
112

28

4

3

2

2
Rabbit
Compatible Secondaries
Clonality
115

35
Polyclonal
Conjugate
84

15

11

5

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This BDNF antibody is un-conjugated
Application
115

50

47

28

25

20

17

13

13

12

6

4

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

B, Hs, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.

Clone

RB14839

Isotype

Ig Fraction

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anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibody
BDNF Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 19-248) antibody
BDNF Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 3D9C5 unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibody
BDNF Reactivity: Pig WB, IHC, IP, ICC Host: Mouse Monoclonal C1 unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 151-247) antibody
BDNF Reactivity: Human, Rat, Mouse WB, ELISA, ICC, IF (cc), IF (p), FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (Internal Region) antibody
Verified BDNF Reactivity: Human ELISA, IF Host: Goat Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) antibody
BDNF Reactivity: Human WB, ELISA, IP, IF Host: Mouse Monoclonal 1B10 unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibody
BDNF Reactivity: Rat, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236) antibody
BDNF Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibody
BDNF Reactivity: Human, Rat, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-247) antibody
BDNF Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Colitti: "Distribution of BDNF and TrkB isoforms in growing antler tissues of red deer." in: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, Vol. 213, pp. 33-46, (2018) (PubMed).
Target

BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name

BDNF (BDNF Products)

Background

BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.

Molecular Weight

27818

Gene ID

627

NCBI Accession

NP_001137277, NP_001137278, NP_001137279, NP_001137280, NP_001137281, NP_001137282, NP_001137283, NP_001137284, NP_001137285, NP_001137286, NP_001137288, NP_001700, NP_733927, NP_733928

UniProt

P23560

Pathways

RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity