beta-2 Microglobulin antibody

Catalog No. ABIN400752

The Mouse Monoclonal anti-beta-2 Microglobulin antibody (Clone 6D585) (ABIN400752) specifically detects beta-2 Microglobulin in WB, FACS, IP, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for beta-2 Microglobulin antibody (ABIN400752)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 6D585

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Recognizes Human beta-2-Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble Human beta-2-Microglobulin. Does not cross-react with Mouse, Bovine, Rabbit, Canine (Dog) or Chicken beta-2-Microglobulin.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin

Purification: DEAE-column Chromatography.

Immunogen: Purified Human beta-2-Microglobulin.

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot: 2-4 μg/mL. Flow Cytometry: 1 μg/mL. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4 containing 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta-2-Microglobulin

Background: Beta-2-Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

Gene ID: 567

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process