FITC antibody (HRP)

Catalog No. ABIN401127

Product Details anti-FITC Antibody (HRP)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This FITC antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)

Purification: Immunoaffinity Chromatography

Immunogen: Fluorescein conjugated to Goat IgG

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Do Not Add Sodium Azide

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-FITC antibody (HRP) (ABIN401127)

Tokuji, Maeda, Yorimitsu, Osuka: "New synthetic strategy for diporphyrins: pinacol coupling-rearrangement." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 17, Issue 26, pp. 7154-7, (2011) (PubMed).

Hamzah, Kotamraju, Seo, Agemy, Fogal, Mahakian, Peters, Roth, Gagnon, Ferrara, Ruoslahti: "Specific penetration and accumulation of a homing peptide within atherosclerotic plaques of apolipoprotein E-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 17, pp. 7154-9, (2011) (PubMed).

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate