NIPBL antibody (AA 344-356)

Catalog No. ABIN401167

Product Details anti-NIPBL Antibody

Target: NIPBL (Nipped-B like Protein (NIPBL))

Binding Specificity: AA 344-356

Reactivity: Human, Mouse, Rat, Dog, Cow, Orang-Utan

Host: Goat

Clonality: Polyclonal

Conjugate: This NIPBL antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against IDN3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Mouse, Canine, Bovine, Orangutan.
Species reactivity (tested):Human.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acids 344-356 of Human IDN3

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2000 - 1/10000. Western Blot: 1/500 - 1/3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NIPBL

Target: NIPBL (Nipped-B like Protein (NIPBL))

Alternative Name: NIPBL (NIPBL Products)

Synonyms: CDLS antibody, CDLS1 antibody, IDN3 antibody, IDN3-B antibody, Scc2 antibody, SCC2W antibody, scc2 antibody, nipbl antibody, scc2-2 antibody, NIPBL antibody, nipbla antibody, wu:fi33d08 antibody, 4921518A06Rik antibody, 4933421G18Rik antibody, C79399 antibody, Idn3 antibody, NIPBL, cohesin loading factor antibody, Nipped-B homolog-like antibody, NIPBL, cohesin loading factor S homeolog antibody, nipped-B homolog b (Drosophila) antibody, nipped-B-like protein A antibody, Nipped-B homolog (Drosophila) antibody, NIPBL antibody, NIPBLL antibody, nipbl.S antibody, LOC427439 antibody, nipblb antibody, LOC100164947 antibody, nipbl antibody, Nipbl antibody

Background: IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion, subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatin during S phase. It shares 37 % sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancerpromoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine. Multiple isoforms of the protein have been reported.Synonyms: Delangin, IDN3, Nipped-B-like protein, SCC2 homolo

Gene ID: 25836

NCBI Accession: NP_056199

UniProt: Q6KC79

Pathways: Sensory Perception of Sound, Stem Cell Maintenance