Transferrin antibody (HRP)

Catalog No. ABIN401186

Product Details anti-Transferrin Antibody (HRP)

Target: Transferrin (TF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Specificity: This antibody is specific for Transferrin (TF).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.

Immunogen: Human Transferrin

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 300 - 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Gentamicin Sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.

Target Details for Transferrin

Target: Transferrin (TF)

Abstract: TF Products

Synonyms: ltf antibody, pro1557 antibody, pro2086 antibody, mgc107777 antibody, LOC692564 antibody, TF antibody, LOC100144362 antibody, tf antibody, LTF antibody, TFEW antibody, conalbumin antibody, tf-b antibody, MGC64306 antibody, PRO1557 antibody, PRO2086 antibody, TFQTL1 antibody, AI266983 antibody, Cd176 antibody, HP antibody, Tf antibody, Tfn antibody, hpx antibody, Trf antibody, cb285 antibody, gavi antibody, id:ibd3238 antibody, id:ibd3525 antibody, sb:cb285 antibody, wu:fb57g06 antibody, wu:fb62h02 antibody, wu:fb63h10 antibody, wu:fb64h10 antibody, zgc:112154 antibody, 143958_at antibody, CG6186 antibody, Dmel\\CG6186 antibody, TSF1 antibody, anon-EST:Posey265 antibody, tsf1 antibody, Pro-TRH antibody, IL-5 antibody, STF I antibody, TRF1 antibody, sTF1 antibody, sTf antibody, tf1 antibody, transferrin antibody, serotransferrin antibody, transferrin (ovotransferrin) antibody, transferrin L homeolog antibody, melanotransferrin antibody, transferrin-a antibody, Transferrin 1 antibody, thyrotropin releasing hormone antibody, interleukin 5 antibody, TF antibody, LOC477072 antibody, tf antibody, Tf antibody, LOC100144362 antibody, tf.L antibody, LOC5575625 antibody, Trf antibody, tfa antibody, Tsf1 antibody, TRH antibody, IL5 antibody, LOC101085148 antibody, LOC100726872 antibody, trf antibody

Background: Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

Gene ID: 7018

NCBI Accession: NP_001054

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis