Transferrin antibody
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- Target See all Transferrin (TF) Antibodies
- Transferrin (TF)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Transferrin antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody detects Transferrin (TF).
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 µm filtered, aseptically filled, and stored at -20°C. An aliquot was submitted for final qualification.
- Immunogen
- Human Transferrin
- Isotype
- IgG
- Top Product
- Discover our top product TF Primary Antibody
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- Application Notes
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ELISA: 1: 12,000 to 1: 43,000, 1.0 μg of Human Transferrin in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] and TMB as asubstrate for 30 minutes at room temperature. Western blot. Immunohistochemistry in frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- Target
- Transferrin (TF)
- Abstract
- TF Products
- Synonyms
- ltf antibody, pro1557 antibody, pro2086 antibody, mgc107777 antibody, LOC692564 antibody, TF antibody, LOC100144362 antibody, tf antibody, LTF antibody, TFEW antibody, conalbumin antibody, tf-b antibody, MGC64306 antibody, PRO1557 antibody, PRO2086 antibody, TFQTL1 antibody, AI266983 antibody, Cd176 antibody, HP antibody, Tf antibody, Tfn antibody, hpx antibody, Trf antibody, cb285 antibody, gavi antibody, id:ibd3238 antibody, id:ibd3525 antibody, sb:cb285 antibody, wu:fb57g06 antibody, wu:fb62h02 antibody, wu:fb63h10 antibody, wu:fb64h10 antibody, zgc:112154 antibody, 143958_at antibody, CG6186 antibody, Dmel\\CG6186 antibody, TSF1 antibody, anon-EST:Posey265 antibody, tsf1 antibody, Pro-TRH antibody, IL-5 antibody, STF I antibody, TRF1 antibody, sTF1 antibody, sTf antibody, tf1 antibody, transferrin antibody, serotransferrin antibody, transferrin (ovotransferrin) antibody, transferrin L homeolog antibody, melanotransferrin antibody, transferrin-a antibody, Transferrin 1 antibody, thyrotropin releasing hormone antibody, interleukin 5 antibody, TF antibody, LOC477072 antibody, tf antibody, Tf antibody, LOC100144362 antibody, tf.L antibody, LOC5575625 antibody, Trf antibody, tfa antibody, Tsf1 antibody, TRH antibody, IL5 antibody, LOC101085148 antibody, LOC100726872 antibody, trf antibody
- Background
- Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin
- Gene ID
- 7018
- NCBI Accession
- NP_001054
- UniProt
- P02787
- Pathways
- Transition Metal Ion Homeostasis
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