PCNA antibody (AA 55-71)

Catalog No. ABIN401315

The Rabbit Polyclonal anti-PCNA antibody is suitable to detect PCNA in samples from Green Algae. It has been validated for WB and EIA.

Quick Overview for PCNA antibody (AA 55-71) (ABIN401315)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Green Algae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PCNA Antibody

Binding Specificity: AA 55-71

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/50,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.33 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: PCNA, or Proliferating Cell Nuclear Antigen, is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA is a nuclear protein.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Gene ID: 37099

UniProt: O22012

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy