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HMGN1 antibody (High Mobility Group Nucleosome Binding Domain 1) (AA 19-28) Primary Antibody

HMGN1 Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Orang-Utan, Pig, Xenopus laevis EIA, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401321
$731.50
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0.1 mg
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Available
  • Target
    HMGN1
    Binding Specificity
    • 17
    • 16
    • 15
    • 15
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 19-28, pSer20, pSer24
    Reactivity
    • 70
    • 15
    • 12
    • 8
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Cow, Dog, Orang-Utan, Pig, Xenopus laevis
    Host
    • 72
    Rabbit
    Clonality
    • 72
    Polyclonal
    Conjugate
    • 30
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This HMGN1 antibody is un-conjugated
    Application
    • 40
    • 31
    • 28
    • 26
    • 26
    • 15
    • 11
    • 6
    • 2
    • 1
    • 1
    Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions). Reactivity is seen against HMGN1 and HMGN2. Reactivity with non-phosphorylated human HMGNs is minimal.
    Cross-Reactivity (Details)
    Species reactivity (expected):Xenopus, Chicken, Mouse, Bovine, Canine, Orangutan, Rat, Porcine.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Immunogen
    Synthetic peptide corresponding to amino acids 19-28 of human HMGN protein
    Isotype
    IgG
  • Application Notes
    ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.20 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    HMGN1
    Alternative Name
    HMGN1 (HMGN1 Antibody Abstract)
    Synonyms
    HMG14, hmg14, hmgn1, hmgn1b, HMG-14, Hmg14, high mobility group nucleosome binding domain 1, high mobility group nucleosome binding domain 1 S homeolog, high mobility group nucleosomal binding domain 1, non-histone chromosomal protein HMG-14, HMGN1, hmgn1.S, hmgn1, Hmgn1, LOC785479
    Background
    HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the HMG-box, that of the HMGN family, the nucleosomal binding domain, and that of the HMGA family, the AThook. The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as HMG motif proteins.Synonyms: HMG-14, HMG-17, HMG14, HMG17, HMGN1, HMGN2, Non-histone chromosomal protein HMG
    Gene ID
    3150
    NCBI Accession
    NP_004956
    UniProt
    P05114
    Pathways
    Chromatin Binding
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