PCNA antibody (Internal Region)

Catalog No. ABIN401372

The Rabbit Polyclonal anti-PCNA antibody is suitable to detect PCNA in samples from Human, Mouse, Rat, Chicken, Monkey, Dog, Cow, Xenopus laevis and Fish. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PCNA antibody (Internal Region) (ABIN401372)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Cow, Xenopus laevis, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PCNA Antibody

Binding Specificity: Internal Region

Specificity: This antibody is directed against PCNA protein.

Cross-Reactivity (Details): Species reactivity (expected):Monkey, Canine, Mouse, Rat, Bovine, Chicken, Fish, Xenopus.
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human PCNA protein

Application Details

Application Notes: ELISA: 1/2,000 - 1/8,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry on Paraffin sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.62 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots)at-20 °C for longer.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Gene ID: 9606

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy