ATM antibody (pSer1981)

Catalog No. ABIN401482

The Sheep Polyclonal anti-ATM antibody is suitable to detect ATM in samples from Human. It has been validated for WB and EIA.

Quick Overview for ATM antibody (pSer1981) (ABIN401482)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ATM Antibody

Binding Specificity: pSer1981

Specificity: This antibody is directed against ATM.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation (IR) for instance) is central to understanding its function. ATM protein is present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. This antibody is similar to the rabbit host antibody discussed by Bakkenist, C. J. & Kastan, M. B. in Nature 421, 499-506 (2003).Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus