His Tag antibody (HRP)

Catalog No. ABIN401743

This Rabbit Polyclonal antibody specifically detects His Tag in WB, IHC (fro) and EIA.

Quick Overview for His Tag antibody (HRP) (ABIN401743)

Target: His Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-His Tag Antibody (HRP)

Sequence: HHHHHH

Immunogen: Synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide

Application Details

Application Notes: ELISA: 1/20,000. Western blot: 1/1,000 - 1/5,000. Immunohistochemistry on frozen sections: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA and 0.01 % (w/v) Gentamicin Sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE.

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.