alpha Fetoprotein antibody

Catalog No. ABIN452558

The Mouse Monoclonal anti-alpha Fetoprotein antibody is suitable to detect alpha Fetoprotein in samples from Human. It has been validated for WB, IP, RIA, EIA, ELISA (Capture) and ELISA (Detection).

Quick Overview for alpha Fetoprotein antibody (ABIN452558)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA), ELISA (Capture), ELISA (Detection)

Clone: AFP-Y2

Product Details anti-alpha Fetoprotein Antibody

Specificity: This antibody reacts with Human AFP. No detectable cross-reaction with Human normal serum proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Purification: Affinity Chromatography on Protein G.

Immunogen: Human AFP, which was isolated from amniotic fluid and purified by AffinityChromatography.

Isotype: IgG1

Application Details

Application Notes: Western blot. Radioimmunoassay. ELISA: Use as Detection antibody with matched AFP Capture antibody clone AFP-Y1(Cat#ABIN452559). Purification of natural Human AFP. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. After reconstitution, aliquot and store at -20 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Background: Alpha Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process