DIO1 antibody (N-Term)

Catalog No. ABIN452912

The Rabbit Polyclonal anti-DIO1 antibody is suitable to detect DIO1 in samples from Human. It has been validated for WB and EIA.

Quick Overview for DIO1 antibody (N-Term) (ABIN452912)

Target: DIO1 (Deiodinase, Iodothyronine, Type I (DIO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIO1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DIO1 Antibody

Binding Specificity: AA 36-66, N-Term

Specificity: This antibody detects DIO1 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of Human DIO1

Application Details

Application Notes: Western blot: 1: 100 - 1: 500. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DIO1

Target: DIO1 (Deiodinase, Iodothyronine, Type I (DIO1))

Alternative Name: DIO1

Background: DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD).Synonyms: DIOI, ITDI1, TXDI1, Type I iodothyronine deiodinase, Type-I 5'-deiodinase

Molecular Weight: 28924 Da

Gene ID: 1733

NCBI Accession: NP_000783

UniProt: P49895

Pathways: Hormone Activity