Dihydrofolate Reductase antibody (C-Term)

Catalog No. ABIN453196

This Rabbit Polyclonal antibody specifically detects Dihydrofolate Reductase in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Dihydrofolate Reductase antibody (C-Term) (ABIN453196)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Dihydrofolate Reductase Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to DHFR / DHFRP1.

Purification: Saturated Ammonium Sulfate (SAS) precipitation

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of mouse DHFR

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR / DHFRP1

Background: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.Synonyms: Dihydrofolate reductase

Gene ID: 13361

NCBI Accession: NP_034179

UniProt: P00375

Pathways: Mitotic G1-G1/S Phases