CD11b antibody

Catalog No. ABIN457402

Product Details anti-CD11b Antibody

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rabbit

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: B10 mouse spleen cells enriched for T cells

Clone: M1-70

Isotype: IgG2b

Application Details

Application Notes: Functional application: In vitro blocking of CD11b.
Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD11b antibody (ABIN457402)

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, (2003) (PubMed).

Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, (2000) (PubMed).

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, (2000) (PubMed).

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, (1981) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Background: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Gene ID: 16409

UniProt: G5E8F1

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation