Mouse anti-Human IgE Antibody (Biotin)
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- Target See all IgE products
- IgE
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
- Specificity
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
- Sensitivity
- Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
- Characteristics
- IGHE, chromosome 14q32.33
- Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
- Purity
- > 85 %, tested by analytical Gel Permeation Chromatography
- Sterility
- Sterile filtered
- Immunogen
- purified human myeloma IgE
- Clone
- BSW17-NBS01
- Isotype
- IgG1 kappa
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- Application Notes
- Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS, pH=7.2, sterile filtered, contains no NaN3
- Preservative
- Azide free
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- Storage
- 4 °C
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- Target
- IgE
- Abstract
- IgE Products
- Background
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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