MLV p30 antibody

Catalog No. ABIN457547

Product Details

Target: MLV p30

Reactivity: Moloney Murine Leukemia Virus (MoMuLV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: MLV Gag, p30

Purification: MLV p30 affinity purified

Immunogen: Full length recombinant MLV-Gag p30 capside protein,purified from E. coli

Isotype: IgG

Application Details

Application Notes: WB 1:1500. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: Concentrated ammonium sulphide in PBS pH 7.4.

Handling Advice: As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use.

Storage: 4 °C

Storage Comment: This product is shipped in non-frozen liquid form in ambient conditions

References

Lee, Komano, Saitoh, Yamaoka, Kozaki, Misawa, Takahama, Satoh, Takeuchi, Yamamoto, Matsuura, Saitoh, Akira: "Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 30, pp. 12379-84, (2013) (PubMed).

Target Details

Target: MLV p30

Target Type: Viral Protein

Background: Murine leukemia viruses belong to family ofRetroviruses group and are named for their ability to cause cancer in mice.Some form of MLV’s are used in cancer research. The retroviral Gag protein plays importantroles at several stages in the viral life cycle. Late in infection, the Gagprecursor protein acts to mediate the assembly and release of virion particles.Afterbudding, the core matures by cleavage of Gag precursor protein intothe mature core proteins, the matrix (MA), capsid (CA) andnucleocapsid proteins. These separated Gag proteins then play roles in the early stages ofinfection