Mouse anti-Human IgM Antibody
-
- Target See all IgM products
- IgM
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgM, isotype specific
- Purification
- Delipidated, heat inactivated lyophilized stable ascites
- Immunogen
- Highly purified monoclonal IgM isolated from pooled human serum
- Clone
- NI 179
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgM, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
- Concentration
- 1.0 mg/mL
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
-
- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
-