alpha-1 Lipoprotein (High-Density-Lipoprotein) antibody
Reactivity: Human
ID, IEP, RID
Host: Goat
Polyclonal
unconjugated
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- Target
- alpha-1 Lipoprotein (High-Density-Lipoprotein)
- Reactivity
- Human
- Host
- Goat
- Clonality
- Polyclonal
- Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- Specificity
- The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
- Characteristics
- Precipitating polyclonal goat antiserum to human alpha-1 lipoprotein (high-density-lipoprotein)
- Purification
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- Immunogen
- Alpha-1 lipoproteins are completely different from beta-lipoproteins. In fresh serum only one alpha-1 lipoprotein exists immunologically, but after centrifugation, storage, freezing and thawing two types of alpha-1 lipoprotein with difference in mobility and antigenic properties have been observed. Alpha-1 lipoprotein in fresh serum is usually precipitated by the specific antiserum as a rather symmetric arc in the albumin zone. Concentration in normal human serum is 170-320 mg/100 ml. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of alpha-1lipoprotein in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:16 when tested against in agar-block immunodiffusion titration against an appropriate
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- Preservative
- Without preservative
- Storage
- 4 °C
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- Target
- alpha-1 Lipoprotein (High-Density-Lipoprotein)
- Background
- The reactivity of the antiserum is restricted to alpha-1 lipoprotein. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-1lipoprotein. No reaction is obtained with any other plasma protein component or serum
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