Serum Proteins Without Immunoglobulins antibody
Reactivity: Human
ID, IEP
Host: Goat
Polyclonal
unconjugated
-
- Target
- Serum Proteins Without Immunoglobulins
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- Specificity
- Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homolo-gous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.
- Characteristics
- Precipitating polyclonal goat antiserum to human serum proteins without immuno-globulins
- Purification
- Adsorption: Immunoaffinity adsorbed using insolubilized immunoglobulins as required. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- Immunogen
- Pooled immunoglobulin-depleted human serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
-
-
- Application Notes
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence of proteins other than immunoglobulins. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the v
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum.
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
-
- Target
- Serum Proteins Without Immunoglobulins
- Background
- Tested in immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipi-tation can be observed of not less than 15 individual proteins components.
-
You are here:
