Serum antibody
Reactivity: Monkey
ID, IEP
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Serum
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Reactivity
- Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- Specificity
- Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species-specific. It will also react with other old-world monkeys, including Cercopithecus, Cynomolgus and baboon. This antiserum is also reacting with chimpanzee, but it gives less precipitin lines in an immunoelectrophoresis than with Rhesus monkey serum.
- Characteristics
- Precipitating polyclonal rabbit antiserum to monkey serum proteins
- Purification
- Adsorption: No adsorption required.
- Immunogen
- Pooled whole Rhesus monkey (Macaca mulatta) serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Target
- Serum
- Alternative Name
- Serum Proteins
- Background
- Tested in immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 16 individual proteins components.
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