Transferrin antibody (Biotin)

Catalog No. ABIN458565

Product Details anti-Transferrin Antibody (Biotin)

Target: Transferrin (TF)

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to swine transferrin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Purified transferrin isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical techniques for the detection of transferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 3.9. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Buffer: Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C , prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin (TF Products)

Synonyms: ltf antibody, pro1557 antibody, pro2086 antibody, mgc107777 antibody, LOC692564 antibody, TF antibody, LOC100144362 antibody, tf antibody, LTF antibody, TFEW antibody, conalbumin antibody, tf-b antibody, MGC64306 antibody, PRO1557 antibody, PRO2086 antibody, TFQTL1 antibody, AI266983 antibody, Cd176 antibody, HP antibody, Tf antibody, Tfn antibody, hpx antibody, Trf antibody, cb285 antibody, gavi antibody, id:ibd3238 antibody, id:ibd3525 antibody, sb:cb285 antibody, wu:fb57g06 antibody, wu:fb62h02 antibody, wu:fb63h10 antibody, wu:fb64h10 antibody, zgc:112154 antibody, 143958_at antibody, CG6186 antibody, Dmel\\CG6186 antibody, TSF1 antibody, anon-EST:Posey265 antibody, tsf1 antibody, Pro-TRH antibody, IL-5 antibody, STF I antibody, TRF1 antibody, sTF1 antibody, sTf antibody, tf1 antibody, transferrin antibody, serotransferrin antibody, transferrin (ovotransferrin) antibody, transferrin L homeolog antibody, melanotransferrin antibody, transferrin-a antibody, Transferrin 1 antibody, thyrotropin releasing hormone antibody, interleukin 5 antibody, TF antibody, LOC477072 antibody, tf antibody, Tf antibody, LOC100144362 antibody, tf.L antibody, LOC5575625 antibody, Trf antibody, tfa antibody, Tsf1 antibody, TRH antibody, IL5 antibody, LOC101085148 antibody, LOC100726872 antibody, trf antibody

Background: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin

Pathways: Transition Metal Ion Homeostasis