Ezrin antibody (AA 352-363)

Catalog No. ABIN461938

Product Details anti-Ezrin Antibody

Target: Ezrin (EZR)

Binding Specificity: AA 352-363

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EZR / Ezrin.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Dog, Horse, Pig (92%) Elephant, Panda, Bat, Bovine, Rabbit, Opossum, Platypus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QDYEEKTKKAER from an internal region of human EZR / Ezrin (NP_003370.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Dog, Horse, Pig (92%), Elephant, Panda, Bat, Bovine, Rabbit, Opossum, Platypus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: EZR / Ezrin (EZR Products)

Synonyms: CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody

Background: Name/Gene ID: EZR

Synonyms: EZR, CVIL, Cytovillin, CVL, p81, VIL2, Villin 2 (ezrin), Villin-2, Cytovillin 2, Ezrin

Gene ID: 7430

NCBI Accession: NP_003370

UniProt: P15311

Pathways: Maintenance of Protein Location