PEBP1 antibody (AA 70-83)

Catalog No. ABIN461941

Product Details anti-PEBP1 Antibody

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Binding Specificity: AA 70-83

Reactivity: Human, Cow, Dog, Monkey, Pig, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PEBP1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Bovine, Dog, Rabbit, Pig (100%) Mouse, Rat, Hamster, Panda, Horse, Opossum, Turkey, Chicken, Catfish, Stickleback, Pike, Pufferfish, Zebrafish, Drosophila, Beetle (93%) Xenopus, Mosquito, Ant, Nematode (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DPDAPSRKDPKYRE from an internal region of human PEBP1 (NP_002558.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Bovine, Dog, Rabbit, Pig (100%), Mouse, Rat, Hamster, Panda, Horse, Opossum, Turkey, Chicken, Catfish, Stickleback, Pike, Pufferfish, Zebrafish, Drosophila, Beetle (93%), Xenopus, Mosquito, Ant, Nematode (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1 / RKIP (PEBP1 Products)

Synonyms: HCNP antibody, HCNPpp antibody, PBP antibody, PEBP antibody, PEBP-1 antibody, RKIP antibody, pbp antibody, hcnp antibody, pebp antibody, rkip antibody, Pbp antibody, Pbp1 antibody, Pbpr antibody, Rkip antibody, PEBP1 antibody, BcDNA:LP12095 antibody, CG18594 antibody, Dmel\\CG18594 antibody, T2 antibody, zgc:56033 antibody, zgc:76942 antibody, phosphatidylethanolamine binding protein 1 antibody, phosphatidylethanolamine binding protein 1 L homeolog antibody, phosphatidylethanolamine-binding protein antibody, phosphatidylethanolamine-binding protein,putative antibody, Phosphatidylethanolamine-binding protein 1 antibody, PEBP1 antibody, pebp1 antibody, Pebp1 antibody, pebp1.L antibody, RR_RS07785 antibody, PVX_235290 antibody, PVX_123630 antibody, PKH_143640 antibody, EAM_1207 antibody

Background: Name/Gene ID: PEBP1

Synonyms: PEBP1, HCNP, HCNPpp, PBP, Prostatic binding protein, Raf kinase inhibitor protein, Raf kinase inhibitory protein, Prostatic-binding protein, Neuropolypeptide h3, PEBP, PEBP-1, RKIP

Gene ID: 5037

NCBI Accession: NP_002558

UniProt: P30086

Pathways: Feeding Behaviour