KCNJ11 antibody (AA 18-30)

Catalog No. ABIN461944

Product Details anti-KCNJ11 Antibody

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Binding Specificity: AA 18-30

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNJ11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KCNJ11 / Kir6.2. This antibody is expected to recognise isoform 1 (NP_000516.3) only.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%), Bovine (85%), Dog (85%), Bat (85%), Horse (85%), Rabbit (85%), Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-AEDPAKPRYRARQ from an internal region (near the N-terminus) of human KCNJ11 / Kir6.2 (NP_000516.3). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%), Bovine (85%), Dog (85%), Bat (85%), Horse (85%), Rabbit (85%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Alternative Name: KCNJ11 / Kir6.2 (KCNJ11 Products)

Synonyms: kir6.2 antibody, Kir6.2 antibody, mBIR antibody, BIR antibody, HHF2 antibody, IKATP antibody, KIR6.2 antibody, PHHI antibody, TNDM3 antibody, potassium voltage-gated channel subfamily J member 11 antibody, potassium inwardly-rectifying channel, subfamily J, member 11 antibody, potassium inwardly rectifying channel, subfamily J, member 11 antibody, KCNJ11 antibody, kcnj11 antibody, Kcnj11 antibody

Background: Name/Gene ID: KCNJ11
Subfamily: Potassium channel - inward-rectifying
Family: Ion Channel

Synonyms: KCNJ11, BIR, IKATP, HHF2, PHHI, TNDM3, Beta-cell KATP channel, KIR6.2

Gene ID: 3767

NCBI Accession: NP_000516

UniProt: Q14654

Pathways: Negative Regulation of Hormone Secretion