Glucose-6-Phosphate Dehydrogenase antibody (AA 305-318)

Catalog No. ABIN461946

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Binding Specificity: AA 305-318

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human G6PD. This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Opossum, Platypus, Lizard, Pufferfish (100%) Galago, Rat, Sheep, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Horse, Xenopus, Trout, Salmon, Stickleback, Zebrafish (93%) Mouse, Shrew, Cat, Medaka (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KPASTNSDDVRDEK from an internal region of human G6PD (NP_000393.4 , NP_001035810.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Opossum, Platypus, Lizard, Pufferfish (100%), Galago, Rat, Sheep, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Horse, Xenopus, Trout, Salmon, Stickleback, Zebrafish (93%), Mouse, Shrew, Cat, Medaka (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000), WB (1:8000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD (G6PD Products)

Synonyms: G6PD1 antibody, g6pd antibody, G6pd antibody, G6pdx antibody, g6pdh antibody, g6pd2 antibody, fj78b06 antibody, wu:fj78b06 antibody, si:dkey-90a13.8 antibody, BA3433 antibody, CORTRD1 antibody, G6PDH antibody, GDH antibody, G28A antibody, G6PD antibody, Gpdx antibody, glucose-6-phosphate dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase (G6PD) antibody, glucose-6-P dehydrogenase antibody, glucose-6-phosphate dehydrogenase L homeolog antibody, glucose-6-phosphate 1-dehydrogenase Zwf antibody, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase antibody, glucose-6-phosphate dehydrogenase X-linked antibody, G6PD antibody, g6pd antibody, g6pD antibody, zwf antibody, LOC100120232 antibody, LACBIDRAFT_188936 antibody, G6pd antibody, g6pd.L antibody, CNG03280 antibody, Tb10.70.5200 antibody, H6PD antibody, G6pdx antibody, H6pd antibody

Background: Name/Gene ID: G6PD

Synonyms: G6PD, G6PD1

Gene ID: 2539

NCBI Accession: NP_000393, NP_001035810

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones