Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Collagen Type I antibody

COL1 Reactivity: Human ELISA, IHC, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN462339
  • Target See all Collagen Type I (COL1) Antibodies
    Collagen Type I (COL1) (Collagen, Type I (COL1))
    Reactivity
    • 97
    • 63
    • 59
    • 20
    • 18
    • 17
    • 15
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 67
    • 36
    • 14
    Rabbit
    Clonality
    • 81
    • 36
    Polyclonal
    Conjugate
    • 57
    • 14
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    This Collagen Type I antibody is un-conjugated
    Application
    • 71
    • 46
    • 38
    • 35
    • 32
    • 27
    • 27
    • 26
    • 20
    • 18
    • 16
    • 8
    • 6
    • 5
    • 5
    • 4
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Brand
    IHC-plus™
    Specificity
    Human collagen type. Cross-reactivity: Human collagen type I: 100 % , Human collagen type II: <0.5 %, Human collagen type III: < 25 %, Human collagen type IV: <0.5 %, Human collagen type V: <0.5 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin: <0.5 %.
    Purification
    Ammonium sulfate precipitation
    Immunogen
    Human placental collagen Type I.

    Type of Immunogen: Purified protein
    Isotype
    IgG
    Top Product
    Discover our top product COL1 Primary Antibody
  • Application Notes
    Approved: ELISA, IF, IHC, IHC-P (1:100)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

    Not recommended for: WB
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Lot specific
    Buffer
    Lyophilized, PBS,  pH 7.5, 0.1 % Mannitol, 0.1 % Dextran.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C,-20 °C
    Storage Comment
    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target
    Collagen Type I (COL1) (Collagen, Type I (COL1))
    Alternative Name
    Collagen I (COL1 Products)
    Synonyms
    collagen type III alpha 1 chain antibody, COL3A1 antibody
You are here:
Support