Integrin beta 2 antibody (AA 534-546)

Catalog No. ABIN462406

This Mouse Monoclonal antibody specifically detects Integrin beta 2 in FACS, WB, IHC, IP, IHC (fro), Func and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Integrin beta 2 antibody (AA 534-546) (ABIN462406)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-48

Product Details anti-Integrin beta 2 Antibody

Binding Specificity: AA 534-546

Brand: IHC-plus™

Specificity: Recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kD type I transmembrane protein expressed on all leukocytes.

No Cross-Reactivity: Dog (Canine)

Purification: Protein A purified

Sterility: 0.2 μm filtered

Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (2 μg/mL), Func, IHC, IHC-Fr, IHC-P (10 μg/mL), IP (2 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 μm filtered

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: ITGB2 / MAC-1 / CD18

Background: Name/Gene ID: ITGB2
Family: Integrin

Synonyms: ITGB2, CD18 antigen, CD18, Integrin beta chain, beta 2, LCAMB, MAC-1, MFI7, MF17, Integrin beta-2, LAD, LFA-1

Gene ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation