PDIA4 antibody (AA 623-638)

Catalog No. ABIN462442

Product Details anti-PDIA4 Antibody

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Binding Specificity: AA 623-638

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Detects an ~72 kD protein, corresponding to the apparent molecular mass of ERp72.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (94%) Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%) Pig, Platypus (81%).

Purification: Protein A purified

Immunogen: A 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (94%), Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%), Pig, Platypus (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for PDIA4

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Alternative Name: PDIA4 / ERP72 (PDIA4 Products)

Synonyms: LOC100229535 antibody, cai antibody, wu:fd20c07 antibody, zgc:113965 antibody, zgc:136625 antibody, zgc:56014 antibody, zgc:77773 antibody, AI987846 antibody, Cai antibody, ERp-72 antibody, Erp72 antibody, ERP70 antibody, ERP72 antibody, Erp70 antibody, protein disulfide isomerase family A member 4 antibody, protein disulfide isomerase family A, member 4 antibody, protein disulfide isomerase associated 4 antibody, protein disulfide isomerase family A member 4 L homeolog antibody, PDIA4 antibody, pdia4 antibody, Pdia4 antibody, pdia4.L antibody

Background: Name/Gene ID: PDIA4

Synonyms: PDIA4, ER protein 70, ERp-72, ERP70, ERP72, Protein disulfide-isomerase A4, ER protein 72

Gene ID: 9601

UniProt: P13667

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis